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Serological diagnosis of dengue infection in blood plasma using long-range surface plasmon waveguides.

Authors :
Wong WR
Krupin O
Sekaran SD
Mahamd Adikan FR
Berini P
Source :
Analytical chemistry [Anal Chem] 2014 Feb 04; Vol. 86 (3), pp. 1735-43. Date of Electronic Publication: 2014 Jan 23.
Publication Year :
2014

Abstract

We present a compact, cost-effective, label-free, real-time biosensor based on long-range surface plasmon polariton (LRSPP) gold (Au) waveguides for the detection of dengue-specific immunoglobulin M (IgM) antibody, and we demonstrate detection in actual patient blood plasma samples. Two surface functionalization approaches are proposed and demonstrated: a dengue virus serotype 2 (DENV-2) functionalized surface to capture dengue-specific IgM antibody in blood plasma and the reverse, a blood plasma functionalized surface to capture DENV-2. The results obtained via these two surface functionalization approaches are comparable to, or of greater quality, than those collected by conventional IgM antibody capture enzyme linked immunosorbent assay (MAC-ELISA). Our second functionalization approach was found to minimize nonspecific binding, thus improving the sensitivity and accuracy of the test. We also demonstrate reuse of the biosensors by regenerating the sensing surface down to the virus (or antibody) level or down to the bare Au.

Details

Language :
English
ISSN :
1520-6882
Volume :
86
Issue :
3
Database :
MEDLINE
Journal :
Analytical chemistry
Publication Type :
Academic Journal
Accession number :
24410440
Full Text :
https://doi.org/10.1021/ac403539k