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Antibacterial activity of serine protease inhibitor 1 from kuruma shrimp Marsupenaeus japonicus.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2014 Jun; Vol. 44 (2), pp. 261-9. Date of Electronic Publication: 2014 Jan 09. - Publication Year :
- 2014
-
Abstract
- Serine protease inhibitors (Serpins) are a large family of protease inhibitors involved in many critical biological processes such as blood coagulation, fibrinolysis, programmed cell death, development, and innate immunity. We identified MjSerp1, a serpin in the kuruma shrimp Marsupenaeus japonicus. The MjSerp1 cDNA has a 1239 bp open reading frame (ORF) that encodes a 412-amino acid protein with a 23 aa signal peptide and a classic serpin domain. MjSerp1 has a calculated molecular mass of 46.3 kDa and a predicted isoelectric point of 5.51. MjSerp1 is mainly expressed in the hepatopancreas and the intestines, and is moderately expressed in hemocytes. Expression pattern analysis indicated that MjSerp1 is upregulated in the hepatopancreas after Vibrio anguillarum challenge. rMjSerp1 inhibits three Gram-positive bacteria and two Gram-negative bacteria, but does not inhibit phenoloxidase activity. The microorganism binding assay showed that rMjSerp1 closely binds to both Gram-positive and Gram-negative bacteria. MjSerp1 also exhibits inhibitory activity against microbial serine proteases, such as subtilisin A and proteinase K, indicating that MjSerp1 acts as a microbial serine protease inhibitor. rMjSerp1 injection into shrimp enhances V. anguillarum clearance, but MjSerp1 knockdown through RNA interference impairs Vibrio clearance in vivo. These results indicate that MjSerp1 functions as a direct effector in the bacterial clearance of M. japonicus. All together, our findings provide novel evidences for the serine protease inhibitor in shrimp immunity.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Anti-Bacterial Agents isolation & purification
Arthropod Proteins genetics
Arthropod Proteins isolation & purification
Arthropod Proteins metabolism
Bacterial Proteins metabolism
Endopeptidase K metabolism
Immunity, Innate
Molecular Sequence Data
RNA, Small Interfering genetics
Recombinant Proteins genetics
Serine Proteinase Inhibitors genetics
Serine Proteinase Inhibitors isolation & purification
Serpins genetics
Serpins isolation & purification
Subtilisins metabolism
Transcriptome
Up-Regulation
Anti-Bacterial Agents metabolism
Artemia immunology
Gram-Positive Bacteria immunology
Gram-Positive Bacterial Infections immunology
Hepatopancreas metabolism
Intestinal Mucosa metabolism
Serine Proteinase Inhibitors metabolism
Serpins metabolism
Vibrio immunology
Vibrio Infections immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 44
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 24412215
- Full Text :
- https://doi.org/10.1016/j.dci.2014.01.002