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NOD1 and NOD2 expression and function in very preterm infant mononuclear cells.

Authors :: Granland C
Strunk T
Hibbert J
Prosser A
Simmer K
Burgner D
Richmond P
Currie AJ
Source :: Acta paediatrica (Oslo, Norway : 1992) [Acta Paediatr] 2014 May; Vol. 103 (5), pp. e212-8. Date of Electronic Publication: 2014 Feb 07.
Publication Year :: 2014
Abstract: Aim: To evaluate mononuclear cell expression and function of the cytosolic nucleotide-binding oligomerization domain-containing receptors, NOD1 and NOD2, in very preterm and full-term infants.<br />Methods: NOD1 and NOD2 gene and protein expression in very preterm infants, term infants and healthy adult, cord and peripheral blood mononuclear cells (C/PBMC) were quantified using qPCR and flow cytometry. Cytokine responses of purified infant and adult monocytes to NOD1- and NOD2-specific agonists were assessed using a multiplex immunoassay (Bioplex).<br />Results: NOD1 and NOD2 were expressed by a range of infant and adult mononuclear cell types, including T- and B cells, with highest expression in classical (CD14(++) CD16(-) ) and intermediate (CD14(++) CD16(+) ) monocytes. NOD1 and NOD2 expression levels by monocytes from very preterm infant were similar to those in term infants or adults. Monocyte production of TNFα, IL-6 and IL-1β induced by activation of NOD1 and NOD2 was similar between very preterm infants, term infants and adults.<br />Conclusion: Monocyte expression and function of NOD1 and NOD2 in very preterm infants are intact and comparable/equivalent to term infants and adults. Functional deficiencies in monocyte NOD signalling pathways are unlikely to contribute to the increased susceptibility to bacterial sepsis in preterm infants.<br /> (©2014 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.)