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The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation.

Authors :
Hoogendoorn E
Crosby KC
Leyton-Puig D
Breedijk RM
Jalink K
Gadella TW
Postma M
Source :
Scientific reports [Sci Rep] 2014 Jan 24; Vol. 4, pp. 3854. Date of Electronic Publication: 2014 Jan 24.
Publication Year :
2014

Abstract

The quality of super resolution images obtained by stochastic single-molecule microscopy critically depends on image analysis algorithms. We find that the choice of background estimator is often the most important determinant of reconstruction quality. A variety of techniques have found use, but many have a very narrow range of applicability depending upon the characteristics of the raw data. Importantly, we observe that when using otherwise accurate algorithms, unaccounted background components can give rise to biases on scales defeating the purpose of super-resolution microscopy. We find that a temporal median filter in particular provides a simple yet effective solution to the problem of background estimation, which we demonstrate over a range of imaging modalities and different reconstruction methods.

Details

Language :
English
ISSN :
2045-2322
Volume :
4
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
24458236
Full Text :
https://doi.org/10.1038/srep03854