Initiation of the flexirubin biosynthesis in Chitinophaga pinensis.

Authors :: Schöner TA
Fuchs SW
Schönau C
Bode HB
Source :: Microbial biotechnology [Microb Biotechnol] 2014 May; Vol. 7 (3), pp. 232-41. Date of Electronic Publication: 2014 Jan 28.
Publication Year :: 2014
Abstract: Bacteria from the Bacteroidetes phylum are known producers of the chemotaxonomic relevant flexirubins. These orange pigments comprise a non-isoprenoid aryl-polyene carboxylic acid esterified with a dialkylresorcinol. Herein, we report a gene cluster from Chitinophaga pinensis encoding the biosynthesis of the polyene moiety and the biochemical characterization of a tyrosine ammonia-lyase and a 4-coumarate-CoA ligase responsible for the initiation of the polyene biosynthesis. Additionally, the flexirubin of C. pinensis was characterized by a combination of feeding experiments, high-performance liquid chromatography tandem mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry.<br /> (© 2014 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.)