A simple chemical synthesis of sugar nucleoside diphosphates in water.

Authors :: Tanaka H
Yoshimura Y
Hindsgaul O
Source :: Current protocols in nucleic acid chemistry [Curr Protoc Nucleic Acid Chem] 2013 Oct 08; Vol. 54, pp. 13.12.1-13.12.10. Date of Electronic Publication: 2013 Oct 08.
Publication Year :: 2013
Abstract: Chemoenzymatic oligosaccharide synthesis is attractive since it eliminates the tedious multistep protection-deprotection requirements of pure chemical synthesis. Chemoenzymatic synthesis using glycosyltransferases, however, requires not only the correct enzyme to control both regio- and stereospecificity, but also the glycosyl donor to provide the sugar that is added. This unit describes a simple synthesis of sugar-nucleoside diphosphates (sugar-NDPs), the type of glycosyl donor (e.g., UDP-Glc, UDP-Gal, ADP-Glc) required by most glycosyltransferases, by using a chemical coupling reaction in water. The preparation of sugar-NDPs by this method therefore does not require any skills in synthetic organic chemistry.<br /> (Copyright © 2013 John Wiley & Sons, Inc.)