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Photodynamic inactivation of multidrug-resistant bacteria in hospital wastewaters: influence of residual antibiotics.
- Source :
-
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2014 Apr; Vol. 13 (4), pp. 626-33. - Publication Year :
- 2014
-
Abstract
- One environmental concern related to hospital effluents is discharge of them without preliminary treatment. Antimicrobial photodynamic inactivation (PDI) may represent an alternative to the traditional expensive, unsafe and not always effective disinfection methods. The main goal of this work was to assess the efficiency of PDI on clinical multidrug-resistant (MDR) bacteria in hospital wastewaters in order to evaluate its potential use in treating hospital effluents. The efficiency of PDI was assessed using a cationic porphyrin as the photosensitizer (PS), four MDR bacteria either in phosphate buffered saline or in filtrated hospital wastewaters. The synergistic effect of PDI and antibiotics (ampicillin and chloramphenicol) was also evaluated, as well as the effect of the surfactant sodium dodecyl sulfate (SDS). The results show the efficient inactivation of MDR bacteria in PBS (reduction of 6-8 log after 270 min of irradiation at 40 W m(-2) with 5.0 μM of PS). In wastewater, the inactivation of the four MDR bacteria was again efficient and the decrease in bacterial survival starts even sooner. A faster decrease in bacterial survival occurred when PDI was combined with the addition of antibiotics, at sub-inhibitory and inhibitory concentrations, but the SDS did not affect the PDI efficiency. It can be concluded that PDI has potential to be an effective alternative for the inactivation of MDR bacteria in hospital wastewaters and that the presence of antibiotics may enhance its effectiveness.
- Subjects :
- Acinetobacter baumannii drug effects
Acinetobacter baumannii radiation effects
Ampicillin pharmacology
Bacterial Load
Chloramphenicol pharmacology
Drug Resistance, Multiple, Bacterial
Drug Synergism
Escherichia coli drug effects
Escherichia coli radiation effects
Hospitals
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa radiation effects
Sodium Dodecyl Sulfate pharmacology
Staphylococcus aureus drug effects
Staphylococcus aureus radiation effects
Surface-Active Agents pharmacology
Time Factors
Anti-Bacterial Agents pharmacology
Photochemical Processes
Photosensitizing Agents pharmacology
Porphyrins pharmacology
Wastewater microbiology
Water Purification methods
Subjects
Details
- Language :
- English
- ISSN :
- 1474-9092
- Volume :
- 13
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
- Publication Type :
- Academic Journal
- Accession number :
- 24519439
- Full Text :
- https://doi.org/10.1039/c3pp50195g