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Axonal control of the adult neural stem cell niche.
- Source :
-
Cell stem cell [Cell Stem Cell] 2014 Apr 03; Vol. 14 (4), pp. 500-11. Date of Electronic Publication: 2014 Feb 20. - Publication Year :
- 2014
-
Abstract
- The ventricular-subventricular zone (V-SVZ) is an extensive germinal niche containing neural stem cells (NSCs) in the walls of the lateral ventricles of the adult brain. How the adult brain's neural activity influences the behavior of adult NSCs remains largely unknown. We show that serotonergic (5HT) axons originating from a small group of neurons in the raphe form an extensive plexus on most of the ventricular walls. Electron microscopy revealed intimate contacts between 5HT axons and NSCs (B1) or ependymal cells (E1) and these cells were labeled by a transsynaptic viral tracer injected into the raphe. B1 cells express the 5HT receptors 2C and 5A. Electrophysiology showed that activation of these receptors in B1 cells induced small inward currents. Intraventricular infusion of 5HT2C agonist or antagonist increased or decreased V-SVZ proliferation, respectively. These results indicate that supraependymal 5HT axons directly interact with NSCs to regulate neurogenesis via 5HT2C.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Blotting, Western
Brain cytology
Brain physiology
Cell Proliferation
Electrophysiology
Immunoenzyme Techniques
Mice
Microscopy, Electron
Neural Stem Cells metabolism
Neurogenesis
Neurons cytology
RNA, Messenger genetics
Raphe Nuclei cytology
Real-Time Polymerase Chain Reaction
Receptor, Serotonin, 5-HT2C chemistry
Reverse Transcriptase Polymerase Chain Reaction
Serotonin Receptor Agonists pharmacology
Axons physiology
Cell Differentiation
Neural Stem Cells cytology
Neurons physiology
Raphe Nuclei physiology
Receptor, Serotonin, 5-HT2C metabolism
Stem Cell Niche
Subjects
Details
- Language :
- English
- ISSN :
- 1875-9777
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cell stem cell
- Publication Type :
- Academic Journal
- Accession number :
- 24561083
- Full Text :
- https://doi.org/10.1016/j.stem.2014.01.014