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[Identification and characterization of enterovirus 71 isolated in Yunnan province].
- Source :
-
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi] 2013 Aug; Vol. 27 (4), pp. 263-5. - Publication Year :
- 2013
-
Abstract
- Objective: Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.<br />Methods: Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.<br />Result: All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000.<br />Conclusion: Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.
- Subjects :
- Animals
China epidemiology
Chlorocebus aethiops
Enterovirus A, Human classification
Enterovirus A, Human genetics
Female
Hand, Foot and Mouth Disease epidemiology
Humans
Male
Mice
Mice, Inbred ICR
Molecular Sequence Data
Phylogeny
Viral Proteins genetics
Enterovirus A, Human isolation & purification
Hand, Foot and Mouth Disease virology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1003-9279
- Volume :
- 27
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
- Publication Type :
- Academic Journal
- Accession number :
- 24579470