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Structure determination of noncanonical RNA motifs guided by ¹H NMR chemical shifts.
- Source :
-
Nature methods [Nat Methods] 2014 Apr; Vol. 11 (4), pp. 413-6. Date of Electronic Publication: 2014 Mar 02. - Publication Year :
- 2014
-
Abstract
- Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating ¹H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-heavy-atom r.m.s. deviation) in 18 cases.
Details
- Language :
- English
- ISSN :
- 1548-7105
- Volume :
- 11
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nature methods
- Publication Type :
- Academic Journal
- Accession number :
- 24584194
- Full Text :
- https://doi.org/10.1038/nmeth.2876