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Whole genome analysis of a community-associated methicillin-resistant Staphylococcus aureus ST59 isolate from a case of human sepsis and severe pneumonia in China.
- Source :
-
PloS one [PLoS One] 2014 Feb 20; Vol. 9 (2), pp. e89235. Date of Electronic Publication: 2014 Feb 20 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- We report a case of necrotizing pneumonia in a young patient caused by community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) in a teaching hospital in the People's Republic of China. The patient had a typical clinical presentation and was successfully treated with antibiotics and intravenous immunoglobulin. A CA-MRSA strain, named SA268, was isolated from the blood of the patient. The isolate was susceptible to most antimicrobial agents, except cephalosporins, penicillins, and β-lactamase inhibitor combinations. Multi-locus sequence typing (MLST) assigned SA268 to ST59, a clone widely spread in eastern Asia. The strain was positive for Panton Valentine Leukocidin (PVL)-encoding genes and SCCmec type V. We sequenced the complete genome of the SA268 isolate. The genome of SA268 was almost identical to that of the Taiwanese ST59 CA-MRSA strains M013 and SA957. However, we observed several differences in gene composition, which included differences in the SCCmec element and several lipoprotein genes that were present in the Taiwanese strains but absent from SA268.
- Subjects :
- Adolescent
Anti-Bacterial Agents administration & dosage
China
Community-Acquired Infections drug therapy
Community-Acquired Infections microbiology
DNA, Bacterial genetics
Humans
Immunoglobulins, Intravenous administration & dosage
Male
Methicillin-Resistant Staphylococcus aureus drug effects
Methicillin-Resistant Staphylococcus aureus isolation & purification
Microbial Sensitivity Tests
Multilocus Sequence Typing
Phylogeny
Pneumonia, Staphylococcal drug therapy
Pneumonia, Staphylococcal microbiology
Sepsis drug therapy
Sepsis microbiology
Sequence Analysis, DNA
Staphylococcal Infections drug therapy
Staphylococcal Infections microbiology
Community-Acquired Infections genetics
Genome, Bacterial genetics
Methicillin-Resistant Staphylococcus aureus genetics
Pneumonia, Staphylococcal genetics
Sepsis genetics
Staphylococcal Infections genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24586619
- Full Text :
- https://doi.org/10.1371/journal.pone.0089235