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Utility of PAX8 mouse monoclonal antibody in the diagnosis of thyroid, thymic, pleural and lung tumours: a comparison with polyclonal PAX8 antibody.
- Source :
-
Histopathology [Histopathology] 2014 Oct; Vol. 65 (4), pp. 465-72. Date of Electronic Publication: 2014 Apr 26. - Publication Year :
- 2014
-
Abstract
- Aims: The purpose of this study was to compare the immunohistochemical staining profiles of PAX8-polyclonal, PAX8-monoclonal, PAX5-monoclonal and PAX6-monoclonal antibodies in several histological types of primary thoracic and thyroid tumours. In addition, we analysed PAX8 mRNA expression by using in-situ hybridization.<br />Methods and Results: We compared polyclonal PAX8 and monoclonal PAX8, PAX5 and PAX6 antibodies in 962 samples (687 lung carcinomas, 40 malignant pleural mesotheliomas, 138 thymic tumours and 97 thyroid tumours) using the tissue microarray technique. Among thyroid tumours, the monoclonal and polyclonal PAX8 antibodies showed a high positive rate (98.0%). Of 167 polyclonal PAX8 antibody-positive tumours, except for thyroid tumours, 54 cases tested positive for PAX5 and/or PAX6 (31 lung carcinomas and 23 thymic tumours). No PAX8 mRNA expression was detected using RNAscope (in-situ hybridization technique) other than in thyroid tumours. A portion of polyclonal PAX8 antibody-positive tumours showed cross-reactivity for PAX5 or PAX6 protein.<br />Conclusions: Monoclonal PAX8 antibody showed high specificity to thyroid tumours and was superior to the polyclonal antibody.<br /> (© 2014 John Wiley & Sons Ltd.)
- Subjects :
- Animals
Biomarkers analysis
Eye Proteins analysis
Eye Proteins immunology
Homeodomain Proteins analysis
Homeodomain Proteins immunology
Humans
In Situ Hybridization
Lung Neoplasms diagnosis
Mice
PAX5 Transcription Factor analysis
PAX5 Transcription Factor immunology
PAX6 Transcription Factor
PAX8 Transcription Factor
Pleural Neoplasms diagnosis
Repressor Proteins analysis
Repressor Proteins immunology
Thymus Neoplasms diagnosis
Tissue Array Analysis
Antibodies, Monoclonal immunology
Paired Box Transcription Factors analysis
Paired Box Transcription Factors immunology
Thoracic Neoplasms diagnosis
Thyroid Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2559
- Volume :
- 65
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Histopathology
- Publication Type :
- Academic Journal
- Accession number :
- 24592933
- Full Text :
- https://doi.org/10.1111/his.12405