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The therapeutic effect of monocyte chemoattractant protein-1 delivered by an electrospun scaffold for hyperglycemia and nephrotic disorders.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2014 Feb 17; Vol. 9, pp. 985-93. Date of Electronic Publication: 2014 Feb 17 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Here, we investigated in diabetic mice the therapeutic effect of monocyte chemoattractant protein-1 (MCP-1), locally delivered by an electrospun scaffold, on transplanted islets. This therapeutic scheme is expected to exert a synergistic effect to ameliorate hyperglycemia and its associated nephrotic disorders. The cumulative amount of MCP-1 released from the scaffold in vitro within a 3-week window was 267.77 ± 32.18 ng, without a compromise in bioactivity. After 8 weeks following the transplantation, the islet population stimulated by MCP-1 was 35.14%± 7.23% larger than the non-stimulated islet population. Moreover, MCP-1 increased concentrations of blood insulin and C-peptide 2 by 49.83%± 5.29% and 43.49%± 9.21%, respectively. Consequently, the blood glucose concentration in the MCP-1 group was significantly lower than that in the control group at week 2 post-surgery. MCP-1 also enhanced the tolerance of sudden oral glucose challenge. The rapid decrease of blood creatinine, urine creatinine, and blood urea nitrogen suggested that the recovery of renal functions compromised by hyperglycemia could also be attributed to MCP-1. Our study shed new light on a synergistic strategy to alleviate hyperglycemia and nephrotic disorders in diabetic patients.
- Subjects :
- Animals
Blood Glucose metabolism
Chemokine CCL2 genetics
Chemokine CCL2 pharmacokinetics
Diabetes Mellitus, Experimental blood
Diabetic Nephropathies physiopathology
Diabetic Nephropathies therapy
Humans
Hyperglycemia blood
Hyperglycemia therapy
Mice
Mice, Inbred C57BL
Mice, Transgenic
Nanomedicine
Tissue Engineering
Tissue Scaffolds chemistry
Chemokine CCL2 administration & dosage
Diabetes Mellitus, Experimental therapy
Drug Delivery Systems
Islets of Langerhans Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 9
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 24600221
- Full Text :
- https://doi.org/10.2147/IJN.S55812