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Diagnostic efficacy of a recombinant cysteine protease of Spirometra erinacei larvae for serodiagnosis of sparganosis.
- Source :
-
The Korean journal of parasitology [Korean J Parasitol] 2014 Feb; Vol. 52 (1), pp. 41-6. Date of Electronic Publication: 2014 Feb 19. - Publication Year :
- 2014
-
Abstract
- The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis was investigated. The recombinant protein (rSepCp-1) has the molecular weight of 23.4 kDa, and strongly reacted with the sparganum positive human or mice sera but not with negative sera by immunoblotting. ELISA with rSepCp-1 protein or sparganum crude antigen (SeC) was evaluated for the serodiagnosis of sparganosis using patient's sera. The sensitivity and specificity of ELISA using rSepCp-1 protein were 95.0% (19/20) and 99.1% (111/112), respectively. In contrast, the sensitivity and specificity of ELISA with SeC were 100% (20/20) and 96.4% (108/112), respectively. Moreover, in experimentally infected mice, the sensitivity and specificity of both ELISA assays were 100% for the detection of anti-sparganum IgG. It is suggested that the rSepCp-1 protein-based ELISA could provide a highly sensitive and specific assay for the diagnosis of sparganosis.
- Subjects :
- Animals
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Escherichia coli genetics
Gene Expression
Humans
Mice
Molecular Weight
Recombinant Proteins chemistry
Recombinant Proteins genetics
Sensitivity and Specificity
Serologic Tests methods
Spirometra genetics
Antigens, Helminth chemistry
Antigens, Helminth genetics
Cysteine Proteases chemistry
Cysteine Proteases genetics
Parasitology methods
Sparganosis diagnosis
Spirometra enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1738-0006
- Volume :
- 52
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The Korean journal of parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 24623880
- Full Text :
- https://doi.org/10.3347/kjp.2014.52.1.41