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Tissue requirements for establishing long-term CD4+ T cell-mediated immunity following Leishmania donovani infection.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2014 Apr 15; Vol. 192 (8), pp. 3709-18. Date of Electronic Publication: 2014 Mar 14. - Publication Year :
- 2014
-
Abstract
- Organ-specific immunity is a feature of many infectious diseases, including visceral leishmaniasis caused by Leishmania donovani. Experimental visceral leishmaniasis in genetically susceptible mice is characterized by an acute, resolving infection in the liver and chronic infection in the spleen. CD4+ T cell responses are critical for the establishment and maintenance of hepatic immunity in this disease model, but their role in chronically infected spleens remains unclear. In this study, we show that dendritic cells are critical for CD4+ T cell activation and expansion in all tissue sites examined. We found that FTY720-mediated blockade of T cell trafficking early in infection prevented Ag-specific CD4+ T cells from appearing in lymph nodes, but not the spleen and liver, suggesting that early CD4+ T cell priming does not occur in liver-draining lymph nodes. Extended treatment with FTY720 over the first month of infection increased parasite burdens, although this associated with blockade of lymphocyte egress from secondary lymphoid tissue, as well as with more generalized splenic lymphopenia. Importantly, we demonstrate that CD4+ T cells are required for the establishment and maintenance of antiparasitic immunity in the liver, as well as for immune surveillance and suppression of parasite outgrowth in chronically infected spleens. Finally, although early CD4+ T cell priming appeared to occur most effectively in the spleen, we unexpectedly revealed that protective CD4+ T cell-mediated hepatic immunity could be generated in the complete absence of all secondary lymphoid tissues.
- Subjects :
- Animals
Antigens, Protozoan immunology
CD4-Positive T-Lymphocytes drug effects
Dendritic Cells immunology
Epitopes, T-Lymphocyte immunology
Female
Fingolimod Hydrochloride
Immunosuppressive Agents pharmacology
Liver drug effects
Liver immunology
Liver parasitology
Lymphocyte Activation immunology
Lymphoid Tissue drug effects
Lymphoid Tissue immunology
Lymphoid Tissue parasitology
Mice
Mice, Knockout
Propylene Glycols pharmacology
Sphingosine analogs & derivatives
Sphingosine pharmacology
Spleen drug effects
Spleen immunology
Spleen parasitology
CD4-Positive T-Lymphocytes immunology
Immunologic Memory
Leishmania donovani immunology
Leishmaniasis, Visceral immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 192
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 24634490
- Full Text :
- https://doi.org/10.4049/jimmunol.1300768