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Monitoring the efficacy of dendritic cell vaccination by early detection of (99m) Tc-HMPAO-labelled CD4(+) T cells.

Authors :
Sharif-Paghaleh E
Leech J
Sunassee K
Ali N
Sagoo P
Lechler RI
Smyth LA
Lombardi G
Mullen GE
Source :
European journal of immunology [Eur J Immunol] 2014 Jul; Vol. 44 (7), pp. 2188-91. Date of Electronic Publication: 2014 Apr 14.
Publication Year :
2014

Abstract

DC vaccines have been used to induce tumour-specific cytotoxic T cells . However, this approach to cancer immunotherapy has had limited success. To be successful, injected DCs need to migrate to the LNs where they can stimulate effector T cells . We and others have previously demonstrated by MRI that tumour antigen-pulsed-DCs labelled ex vivo with superparamagnetic iron oxide nanoparticles migrated to the draining LNs and are capable of activating antigen-specific T cells . The results from our study demonstrated that ex vivo superparamagnetic iron oxide nanoparticles-labelled and OVA-pulsed DCs prime cytotoxic CD8(+) T-cell responses to protect against a B16-OVA tumour challenge. In the clinic, a possible noninvasive surrogate marker for efficacy of DC vaccination is to image the specific migration and accumulation of T cells following DC vaccination.<br /> (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Details

Language :
English
ISSN :
1521-4141
Volume :
44
Issue :
7
Database :
MEDLINE
Journal :
European journal of immunology
Publication Type :
Academic Journal
Accession number :
24643793
Full Text :
https://doi.org/10.1002/eji.201344337