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Peyer's patches play a protective role in nonsteroidal anti-inflammatory drug-induced enteropathy in mice.
- Source :
-
Inflammatory bowel diseases [Inflamm Bowel Dis] 2014 May; Vol. 20 (5), pp. 790-9. - Publication Year :
- 2014
-
Abstract
- Background: Peyer's patches (PPs) play a major role in mucosal immunity. However, their roles in nonsteroidal anti-inflammatory drug-induced enteropathy are poorly understood.<br />Methods: Wild-type (WT) and PP-null mice were injected with indomethacin. Twenty-four hours later, the cellular profiles and cytokine levels in the PPs, mesenteric lymph nodes (MLNs), and lamina propria (LP) of the small intestine were measured. WT and PP-null mice were given antibiotics before indomethacin treatment to evaluate enteropathy. Naive CD4 T cells were co-cultured with CD103 or CD103 dendritic cells (DCs) to analyze the interleukin (IL)-10 expression levels. Finally, WT mice adoptively transferred with CD103 or CD103 DCs were injected with indomethacin.<br />Results: The proportion of CD103 DCs in PPs and MLNs and IL-10-expressing CD4 T cells of PPs and the LP increased after indomethacin treatment. The PP-null mice showed greater indomethacin-induced enteropathy, fewer CD103 DCs in their MLNs, and lower proportion of IL-10-expressing CD4 T cells of their LP than WT mice, regardless of commensal bacteria. Naive splenic CD4 T cells co-cultured with CD103 DCs isolated from the MLNs of indomethacin-injected WT mice produced a higher amount of IL-10 compared with those co-cultured with CD103 DCs. Moreover, WT mice that received CD103 DCs showed milder enteropathy than those that received CD103 DCs.<br />Conclusions: PPs play a protective role in nonsteroidal anti-inflammatory drug-induced enteropathy, and this protection is associated with an increase in CD103 DCs and IL-10-producing CD4 T cells in the intestine, independent of the commensal bacteria.
- Subjects :
- Animals
CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes metabolism
Cell Differentiation
Cytokines genetics
Cytokines metabolism
Dendritic Cells cytology
Dendritic Cells immunology
Dendritic Cells metabolism
Female
Flow Cytometry
Interleukin-10 physiology
Intestinal Diseases chemically induced
Intestine, Small drug effects
Intestine, Small pathology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Spleen cytology
Spleen immunology
Spleen metabolism
Anti-Inflammatory Agents, Non-Steroidal toxicity
Indomethacin toxicity
Intestinal Diseases immunology
Intestinal Diseases prevention & control
Intestine, Small immunology
Peyer's Patches immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4844
- Volume :
- 20
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Inflammatory bowel diseases
- Publication Type :
- Academic Journal
- Accession number :
- 24651581
- Full Text :
- https://doi.org/10.1097/MIB.0000000000000017