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Kidney injury molecule-1 is involved in the chemotactic migration of mesenchymal stem cells.
- Source :
-
In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2014 Aug; Vol. 50 (7), pp. 648-55. Date of Electronic Publication: 2014 Mar 21. - Publication Year :
- 2014
-
Abstract
- A better understanding of the organ specific factors that regulate the migration of mesenchymal stem cells (MSCs) into the target organ is essential for optimization of strategies to improve the repair after injury. In the present study, we showed that the kidney injury molecule-1 (KIM-1), a well-known kidney-specific biomarker, enhanced the in vitro migration capacity of MSCs as a potent kidney-specific chemo-attractant or an inducer. The in vitro roles were verified by migration assay using KIM1-PK1 cell lines, the mouse proximal tubular epithelial cells (mPTEs) and recombinant human KIM-1 proteins (rhKIM-1). Immunofluorescence staining displayed specific ectodomain binding of KIM-1 on the surface of MSCs. Upregulation of chemokine receptor type 4 (CXCR4) protein when treated with tumor necrosis factor alpha (TNF-α) was shown. The effect of KIM-1 on migration of MSCs was augmented by TNF-α pretreatment in a dose-dependent manner, and reduced by AMD3100, an antagonist of CXCR4. These results suggest that KIM-1 is a potential chemo-ligand of CXCR4 and may play an important role in kidney-specific migration of MSCs via interaction between KIM-1 and CXCR4.
- Subjects :
- Analysis of Variance
Animals
Benzylamines
Blotting, Western
Cyclams
Dose-Response Relationship, Drug
Flow Cytometry
Fluorescent Antibody Technique
Hepatitis A Virus Cellular Receptor 1
Heterocyclic Compounds
Humans
Mesenchymal Stem Cells metabolism
Mice
Receptors, CXCR4 antagonists & inhibitors
Receptors, CXCR4 metabolism
Tumor Necrosis Factor-alpha pharmacology
Cell Movement physiology
Chemotaxis physiology
Membrane Glycoproteins metabolism
Mesenchymal Stem Cells physiology
Receptors, Virus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1543-706X
- Volume :
- 50
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- In vitro cellular & developmental biology. Animal
- Publication Type :
- Academic Journal
- Accession number :
- 24652046
- Full Text :
- https://doi.org/10.1007/s11626-013-9731-0