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Inhibition of Helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by aqueous extracts and pectic polysaccharides from the roots of Cochlospermum tinctorium A. Rich. and Vernonia kotschyana Sch. Bip. ex Walp.
- Source :
-
Fitoterapia [Fitoterapia] 2014 Jun; Vol. 95, pp. 127-32. Date of Electronic Publication: 2014 Mar 19. - Publication Year :
- 2014
-
Abstract
- In Malian traditional medicine infusions of the roots of Vernonia kotschyana or Cochlospermum tinctorium in water are used for treating gastric ulcer. Helicobacter pylori is known to play a major role in gastric ulcer development, and it was of interest to evaluate a potential anti-adhesive activity towards H. pylori by crude water extracts and isolated polysaccharide fractions from the roots of V. kotschyana and C. tinctorium. The inhibitory effects were examined by an in vitro flow cytometric assay using human gastric adenocarcinoma epithelial cells, where fluorescent-labeled H. pylori were pre-treated with the test fractions. The crude extract Ctw50 from C. tinctorium, containing a mixture of inulin, pectic polysaccharides, phenols and protein, led to a 43% reduction of bacterial attachment. The isolated pectic type fractions CtwA1 and CtwA2 from C. tinctorium, and Vko-I from V. kotschyana resulted in approximately 30% inhibition of H. pylori adhesion. These fractions consist of rhamnogalacturonan backbones with side chains of arabinogalactans and/or arabinans. The low degree of uronic acids in the fractions compared to anti-adhesive polysaccharides reported previously, suggests that the neutral side chains might play a role in the binding of bacterial adhesins. The fraction Vko-III.1 from V. kotschyana consisting mainly of galacturonic acid resulted only in a 19% inhibition of H. pylori adhesion. The anti-adhesive properties shown by the crude water extracts and isolated polysaccharide fractions in the present study might partly explain the anti-ulcer activities by the roots of V. kotschyana and C. tinctorium.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Cell Adhesion drug effects
Epithelial Cells drug effects
Galactans chemistry
Galactans isolation & purification
Galactans pharmacology
Humans
Medicine, Traditional
Pectins chemistry
Pectins isolation & purification
Pectins pharmacology
Phenols chemistry
Phenols isolation & purification
Phenols pharmacology
Plant Extracts chemistry
Plant Extracts isolation & purification
Plant Roots chemistry
Polysaccharides chemistry
Polysaccharides isolation & purification
Polysaccharides pharmacology
Adenocarcinoma microbiology
Bixaceae chemistry
Helicobacter pylori drug effects
Plant Extracts pharmacology
Stomach Ulcer microbiology
Vernonia chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6971
- Volume :
- 95
- Database :
- MEDLINE
- Journal :
- Fitoterapia
- Publication Type :
- Academic Journal
- Accession number :
- 24657817
- Full Text :
- https://doi.org/10.1016/j.fitote.2014.03.009