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In vitro correction of a novel splicing alteration in the BTK gene by using antisense morpholino oligonucleotides.

Authors :
Rattanachartnarong N
Tongkobpetch S
Chatchatee P
Daengsuwan T
Ittiwut C
Suphapeetiporn K
Shotelersuk V
Source :
Archivum immunologiae et therapiae experimentalis [Arch Immunol Ther Exp (Warsz)] 2014 Oct; Vol. 62 (5), pp. 431-6. Date of Electronic Publication: 2014 Mar 23.
Publication Year :
2014

Abstract

A novel sequence variant, c.240+109C>A, in the Bruton's tyrosine kinase (BTK) gene was identified in a patient with X-linked agammaglobulinemia. This alteration resulted in an incorporation of 106 nucleotides of BTK intron 3 into its mRNA. Administration of the 25-mer antisense morpholino oligonucleotide analog in the patient's cultured peripheral blood mononuclear cells was able to restore correctly spliced BTK mRNA, a potential treatment for X-linked agammaglobulinemia.

Details

Language :
English
ISSN :
1661-4917
Volume :
62
Issue :
5
Database :
MEDLINE
Journal :
Archivum immunologiae et therapiae experimentalis
Publication Type :
Academic Journal
Accession number :
24658450
Full Text :
https://doi.org/10.1007/s00005-014-0283-0