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microRNA-200a inhibits cell proliferation by targeting mitochondrial transcription factor A in breast cancer.
- Source :
-
DNA and cell biology [DNA Cell Biol] 2014 May; Vol. 33 (5), pp. 291-300. Date of Electronic Publication: 2014 Mar 31. - Publication Year :
- 2014
-
Abstract
- microRNAs (miRNAs) are endogenous 19-25 nucleotide noncoding single-stranded RNAs that regulate gene expression by blocking the translation or decreasing the stability of mRNAs. In this study, we showed that miR-200a expression levels were decreased while mitochondrial transcription factor A (TFAM) mRNA expression levels were increased in breast cancer (BC) tissues and cell lines, and identified TFAM as a novel direct target of miR-200a. Overexpression of miR-200a suppressed TFAM protein expression, mtDNA copy number, and attenuated cell proliferation. Forced expression of TFAM can partly rescue the inhibitory effect of miR-200a in the cells. Taken together, these findings will shed light on the role and mechanism of miR-200a in regulating BC cells growth and mtDNA copy number via miR-200a/TFAM axis, and miR-200a may serve as a potential therapeutic target in BC in the future.
- Subjects :
- Breast Neoplasms pathology
DNA-Binding Proteins genetics
Female
Gene Expression Regulation, Neoplastic
Humans
MCF-7 Cells
MicroRNAs genetics
Mitochondrial Proteins genetics
Protein Binding
Transcription Factors genetics
Breast Neoplasms metabolism
Cell Proliferation
DNA-Binding Proteins metabolism
MicroRNAs metabolism
Mitochondrial Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1557-7430
- Volume :
- 33
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- DNA and cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 24684598
- Full Text :
- https://doi.org/10.1089/dna.2013.2132