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Nitric oxide stimulates glutamatergic synaptic inputs to baroreceptor neurons through potentiation of Cav2.2-mediated Ca(2+) currents.
- Source :
-
Neuroscience letters [Neurosci Lett] 2014 May 01; Vol. 567, pp. 57-62. Date of Electronic Publication: 2014 Mar 28. - Publication Year :
- 2014
-
Abstract
- Nitric oxide (NO) increases glutamate release to the second-order neurons in the nucleus tractus solitarius (NTS). N-type Ca(2+) channel is essential for triggering glutamate release at synaptic terminals. In this study, we determined the role of Cav2.2 subunit in NO-induced increase in glutamate synaptic inputs to NTS neurons. The second-order NTS neurons and nodose ganglionic (NG) neurons were identified by applying DiA, a fluorescent lipophilic tracer, on aortic depressor nerve in rats. NO donor DEA/NO significantly increased tractus solitarius (TS)-evoked excitatory postsynaptic currents (EPSCs) in second-order NTS neurons, an effect was abolished by pretreatment of slice with ODQ, an inhibitor for soluble isoform of guanylyl cyclase. DEA/NO decreased the paired-pulse ratio of TS-evoked EPSCs, while increased the frequency, but not the amplitude, of miniature EPSCs in second-order NTS neurons. Furthermore, DEA/NO significantly increased Ba(2+) currents in identified baroreceptor NG neurons. However, DEA/NO had little effect on the Ba(2+) currents in the presence of specific N-type Ca(2+) blocker ω-conotoxin GVIA. In addition, immunocytochemistry staining revealed that Cav2.2 subunit immunoreactivates were colocalized with DiA-labeled baroreceptor nerve terminals in the NTS. Collectively, these findings suggest that NO stimulates glutamatergic synaptic inputs to second-order NTS neurons through augmentation of Cav2.2-mediated N-type Ca(2+) currents.<br /> (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Excitatory Postsynaptic Potentials
Hydrazines pharmacology
In Vitro Techniques
Male
Miniature Postsynaptic Potentials
Neurons drug effects
Nitric Oxide Donors pharmacology
Rats, Sprague-Dawley
Solitary Nucleus cytology
Solitary Nucleus drug effects
Solitary Nucleus metabolism
Calcium physiology
Calcium Channels, N-Type physiology
Glutamic Acid metabolism
Neurons physiology
Nitric Oxide metabolism
Pressoreceptors physiology
Synapses physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7972
- Volume :
- 567
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 24686191
- Full Text :
- https://doi.org/10.1016/j.neulet.2014.03.036