Microfluidic perfusion cell culture system confined in 35 mm culture dish for standard biological laboratories.

Authors :: Kondo E
Wada K
Hosokawa K
Maeda M
Source :: Journal of bioscience and bioengineering [J Biosci Bioeng] 2014 Sep; Vol. 118 (3), pp. 356-8. Date of Electronic Publication: 2014 Mar 30.
Publication Year :: 2014
Abstract: An extremely simple, self-standing microfluidic cell culture system is reported. The whole system is confined in a 35 mm culture dish, and requires only a standard CO2 incubator. The culture medium is perfused by gravity. We successfully cultured NIH3T3-derived cells up to 10 days with a viability of ∼90%.<br /> (Copyright © 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)

Subjects :: Animals
Cell Culture Techniques standards
Cell Proliferation
Culture Media
Mice
Microfluidic Analytical Techniques standards
NIH 3T3 Cells
Perfusion methods
Cell Culture Techniques instrumentation
Microfluidic Analytical Techniques instrumentation

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