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N,N'-dinitrosopiperazine-mediated AGR2 is involved in metastasis of nasopharyngeal carcinoma.
- Source :
-
PloS one [PLoS One] 2014 Apr 09; Vol. 9 (4), pp. e92081. Date of Electronic Publication: 2014 Apr 09 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Nasopharyngeal carcinoma (NPC) has a high metastatic character in the clinic, but its mechanism is not clear. As a carcinogen with organ specificity for the nasopharyngeal epithelium, N,N'-Dinitrosopiperazine (DNP) is involved in NPC metastasis. Herein, our data revealed that anterior gradient 2 (AGR2) was overexpressed in human NPC tissues, particularly in cervical lymph node metastatic NPC (LMNPC). High AGR2 expression was associated with NPC metastasis. Importantly, DNP induced AGR2 expression, and increased cell motility and invasion in the NPC cell line 6-10B. However, DNP-mediated cell motility and invasion was dramatically decreased when transfected with siRNA-AGR2. Further, AGR2 directly regulated cathepsin (CTS) B and D by binding them in vitro. These results indicate that DNP induces AGR2 expression, regulates CTSB and CTSD, increases cell motility and invasion, and promotes NPC tumor metastasis. Therefore, DNP-mediated AGR2 expression may be an important factor in prolific NPC metastasis.
- Subjects :
- Carcinoma
Cathepsin B metabolism
Cathepsin D metabolism
Cell Line, Tumor
Cell Movement
Epithelium metabolism
Epithelium pathology
Humans
Immunohistochemistry
Mucoproteins
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms enzymology
Neoplasm Invasiveness
Oncogene Proteins
Protein Binding
RNA, Small Interfering metabolism
Lymphatic Metastasis pathology
Nasopharyngeal Neoplasms metabolism
Nasopharyngeal Neoplasms pathology
Nitrosamines adverse effects
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24717913
- Full Text :
- https://doi.org/10.1371/journal.pone.0092081