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The production of the eosinophil proteins ECP and EPX/EDN are regulated in a reciprocal manner.
- Source :
-
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2014 Apr; Vol. 122 (4), pp. 283-91. - Publication Year :
- 2014
-
Abstract
- Previous studies showed that the biological activity and the eosinophil content of eosinophil cationic protein (ECP, RNase 3) are determined by single-nucleotide polymorphisms (SNPs) in the ECP (RNase3) gene. In this study, we report the prevalence of a common SNP in the eosinophil protein x/eosinophil-derived neurotoxin (EPX/EDN, RNase2) and the association with the cellular contents of EPX/EDN and ECP. The genes were sequenced and the EPX/EDN405(G>C) rs2013109 SNPs were also determined by TaqMan 5′nuclease allelic discrimination assay. ECP and EPX/EDN in purified eosinophils or in whole blood extracts were analysed by sensitive immunoassays. The study included 379 non-allergic and allergic subjects. The genotype prevalence of the EPX/EDN405(G>C) polymorphism was GG 59%, GC 36% and CC 6%. The cellular contents of ECP and EPX/EDN were related in a reciprocal fashion with the sums of the protein contents being constant. The contents were associated with the ECP562(G>C) rs2233860 and EPX/EDN405(G>C) gene polymorphisms. The cellular content of eosinophil peroxidase (EPO) was not associated with the ECP and EPX/EDN genotypes. The prevalence of the EPX/EDN405(G>C) genotypes and the contents of the proteins were similar in non-allergic and allergic subjects.The production and storage of the two ancestral proteins, ECP and EPX/EDN likely share common regulatory mechanisms, which result in opposing productions of the two proteins.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Child
Eosinophil Cationic Protein immunology
Eosinophil-Derived Neurotoxin immunology
Eosinophils enzymology
Eosinophils immunology
Female
Gene Expression Regulation
Gene Frequency
Humans
Hypersensitivity enzymology
Hypersensitivity genetics
Hypersensitivity immunology
Male
Middle Aged
Polymorphism, Single Nucleotide
Young Adult
Eosinophil Cationic Protein biosynthesis
Eosinophil Cationic Protein genetics
Eosinophil-Derived Neurotoxin biosynthesis
Eosinophil-Derived Neurotoxin genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0463
- Volume :
- 122
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 24738159
- Full Text :
- https://doi.org/10.1111/apm.12142