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Full-Genome Sequencing of Four Bluetongue Virus Serotype 11 Viruses.

Authors :: Vandenbussche F
Sailleau C
Rosseel T
Desprat A
Viarouge C
Richardson J
Eschbaumer M
Hoffmann B
De Clercq K
Bréard E
Zientara S
Source :: Transboundary and emerging diseases [Transbound Emerg Dis] 2015 Oct; Vol. 62 (5), pp. 565-71. Date of Electronic Publication: 2013 Oct 08.
Publication Year :: 2015
Abstract: Recently, a contamination incident was described in which the challenge inoculum used in a bluetongue virus serotype 8 (BTV-8) vaccination trial was contaminated with a BTV-11 virus that was closely related to the Belgian BTV-11 virus from 2008. This study reports the first complete genome sequences of four BTV-11 viruses: the BTV-11 contaminant, BTV-11 reference strain, BTV-11 vaccine strain and a recently isolated BTV-11 field strain from Martinique. Full-genome analysis showed that these viruses belong to serotype 11/nucleotype A and cluster together with other western topotype bluetongue viruses. Detailed comparisons of the genomes further indicated that the contaminant was derived from the BTV-11 reference strain, as they were distinguished by a single synonymous nucleotide substitution. The previously reported partial sequence of genome segment 2 of the Belgian BTV-11 was found to be identical to that of the BTV-11 vaccine strain, indicating that it most likely was the BTV-11 vaccine strain. These findings also suggest that the BTV-11 contaminant and the Belgian BTV-11 are not the same viruses. Finally, comparison of the reference and vaccine strain did not allow determining the amino acid substitutions that contribute to the attenuated phenotype.<br /> (© 2013 Blackwell Verlag GmbH.)