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Sample sequencing of vascular plants demonstrates widespread conservation and divergence of microRNAs.
- Source :
-
Nature communications [Nat Commun] 2014 Apr 23; Vol. 5, pp. 3722. Date of Electronic Publication: 2014 Apr 23. - Publication Year :
- 2014
-
Abstract
- Small RNAs are pivotal regulators of gene expression that guide transcriptional and post-transcriptional silencing mechanisms in eukaryotes, including plants. Here we report a comprehensive atlas of sRNA and miRNA from 3 species of algae and 31 representative species across vascular plants, including non-model plants. We sequence and quantify sRNAs from 99 different tissues or treatments across species, resulting in a data set of over 132 million distinct sequences. Using miRBase mature sequences as a reference, we identify the miRNA sequences present in these libraries. We apply diverse profiling methods to examine critical sRNA and miRNA features, such as size distribution, tissue-specific regulation and sequence conservation between species, as well as to predict putative new miRNA sequences. We also develop database resources, computational analysis tools and a dedicated website, http://smallrna.udel.edu/. This study provides new insights on plant sRNAs and miRNAs, and a foundation for future studies.
- Subjects :
- Phylogeny
Plants classification
MicroRNAs genetics
Plants genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 24759728
- Full Text :
- https://doi.org/10.1038/ncomms4722