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Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2014 Jul; Vol. 10 (1), pp. 89-94. Date of Electronic Publication: 2014 Apr 24. - Publication Year :
- 2014
-
Abstract
- Cucurbitacin B (CuB) is a tetracyclic triterpene that is contained in extracts from cucurbitaceous plants and has been demonstrated to have anticancer and anti‑inflammatory activities. The purpose of the present study was to determine whether CuB exhibits anticancer effects on SH‑SY5Y human neuroblastoma cells and to analyze the underlying molecular mechanism. The results demonstrated that CuB not only induced cell cycle arrest at the G2/M phase, but also induced apoptosis as characterized by positive Annexin V staining, downregulation of phospho‑Janus kinase 2 (p‑JAK2), phospho‑signal transducer and activator of transcription 3 (p‑STAT3), phospho‑extracellular signal‑regulated kinases and the activation of c‑Jun N‑terminal kinase and p38 mitogen activated protein kinase (MAPK). CuB also altered the expression of gene products that mediated cell proliferation (Cyclin B1 and cyclin‑dependent kinase 1), cell survival (B‑cell lymphoma 2, Bcl2‑associated X protein) and increased the expression of p53 and p21. These results provide the evidence that JAK2/STAT3 and MAPKs have crucial roles in CuB‑induced growth inhibition and apoptosis in SH‑SY5Y human neuroblastoma cells.
- Subjects :
- Antineoplastic Agents pharmacology
Cell Line, Tumor
Cell Proliferation drug effects
G2 Phase Cell Cycle Checkpoints drug effects
Humans
M Phase Cell Cycle Checkpoints drug effects
Neuroblastoma metabolism
Neuroblastoma pathology
Phosphorylation drug effects
Proto-Oncogene Proteins c-bcl-2 metabolism
Signal Transduction drug effects
bcl-2-Associated X Protein metabolism
Apoptosis drug effects
Extracellular Signal-Regulated MAP Kinases metabolism
Janus Kinase 2 metabolism
STAT3 Transcription Factor metabolism
Triterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 24789581
- Full Text :
- https://doi.org/10.3892/mmr.2014.2175