Back to Search
Start Over
Targeting inhibitory chondroitin sulphate proteoglycans to promote plasticity after injury.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1162, pp. 127-38. - Publication Year :
- 2014
-
Abstract
- Chondroitin sulphate proteoglycans (CSPGs) are one of the major families of inhibitory extracellular matrix molecules in the central nervous system. The expression of various CSPGs is strong during early nervous system development; however, it is downregulated during maturation and up-regulated again after nervous system injury. In vivo injection of an enzyme called chondroitinase ABC, which removes the inhibitory chondroitin sulphate chains on the CSPGs, in the injured area promotes both the regeneration and plasticity of the neurons. Here, we describe the method of in vivo injection of the chondroitinase ABC into the cortex of adult rat brain and the histochemical method to assess the successfulness of the digestion.
- Subjects :
- Anesthesia methods
Animals
Brain metabolism
Brain pathology
Brain physiopathology
Chondroitin ABC Lyase administration & dosage
Enzyme Therapy methods
Histocytochemistry methods
Injections
Male
Molecular Targeted Therapy methods
Perfusion methods
Rats
Rats, Sprague-Dawley
Brain drug effects
Chondroitin ABC Lyase therapeutic use
Chondroitin Sulfate Proteoglycans metabolism
Neuronal Plasticity drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1162
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 24838963
- Full Text :
- https://doi.org/10.1007/978-1-4939-0777-9_10