Back to Search
Start Over
Shotgun proteomics: identification of unique protein profiles of apoptotic bodies from biliary epithelial cells.
- Source :
-
Hepatology (Baltimore, Md.) [Hepatology] 2014 Oct; Vol. 60 (4), pp. 1314-23. Date of Electronic Publication: 2014 Jul 31. - Publication Year :
- 2014
-
Abstract
- Unlabelled: Shotgun proteomics is a powerful analytic method to characterize complex protein mixtures in combination with multidimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS). We used this platform for proteomic characterization of apoptotic bodies in an effort to define the complex protein mixtures found in primary cultures of human intrahepatic biliary epithelial cells (HiBEC), human renal proximal tubular epithelial cells, human bronchial epithelial cells, isolated intrahepatic biliary epithelial cells from explanted primary biliary cirrhosis (PBC), and control liver using a total of 24 individual samples. Further, as additional controls and for purposes of comparison, proteomic signatures were also obtained from intact cells and apoptotic bodies. The data obtained from LC-MS/MS, combined with database searches and protein assembly algorithms, allowed us to address significant differences in protein spectral counts and identify unique pathways that may be a component of the induction of the signature inflammatory cytokine response against BECs, including the Notch signaling pathway, interleukin (IL)8, IL6, CXCR2, and integrin signaling. Indeed, there are 11 proteins that localize specifically to apoptotic bodies of HiBEC and eight proteins that were specifically absent in HiBEC apoptotic bodies.<br />Conclusion: Proteomic analysis of BECs from PBC liver compared to normal liver are significantly different, suggesting that an immunological attack affects the repertoire of proteins expressed and that such cells should be thought of as living in an environment undergoing continuous selection secondary to an innate and adaptive immune response, reflecting an almost "Darwinian" bias.<br /> (© 2014 by the American Association for the Study of Liver Diseases.)
- Subjects :
- Adaptive Immunity
Bile Ducts, Intrahepatic pathology
Bronchi metabolism
Bronchi pathology
Case-Control Studies
Cells, Cultured
Chromatography, Liquid
Epithelial Cells pathology
Humans
Immunity, Innate
Kidney Tubules, Proximal metabolism
Kidney Tubules, Proximal pathology
Liver metabolism
Liver pathology
Liver Cirrhosis, Biliary pathology
Tandem Mass Spectrometry
Apoptosis
Bile Ducts, Intrahepatic metabolism
Epithelial Cells metabolism
Liver Cirrhosis, Biliary metabolism
Protein Array Analysis methods
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1527-3350
- Volume :
- 60
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Hepatology (Baltimore, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 24841946
- Full Text :
- https://doi.org/10.1002/hep.27230