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Acceleration of bone union after structural bone grafts with a collagen-binding basic fibroblast growth factor anchored-collagen sheet for critical-size bone defects.
- Source :
-
Biomedical materials (Bristol, England) [Biomed Mater] 2014 Jun; Vol. 9 (3), pp. 035014. Date of Electronic Publication: 2014 May 20. - Publication Year :
- 2014
-
Abstract
- Bone allografts are commonly used for the repair of critical-size bone defects. However, the loss of cellular activity in processed grafts markedly reduces their healing potential compared with autografts. To overcome this obstacle, we developed a healing system for critical-size bone defects that consists of overlaying an implanted bone graft with a collagen sheet (CS) loaded with basic fibroblast growth factor (bFGF) fused to the collagen-binding domain derived from a Clostridium histolyticum collagenase (CB-bFGF). In a murine femoral defect model, defect sites treated with CS/CB-bFGF had a significantly larger callus volume than those treated with CS/native bFGF. In addition, treatment with CS/CB-bFGF resulted in the rapid formation of a hard callus bridge and a larger total callus volume at the host-graft junction than treatment with CS/bFGF. Our results suggest that the combined use of CS and CB-bFGF helps accelerate the union of allogenic bone grafts.
- Subjects :
- Animals
Bone Transplantation methods
Drug Implants administration & dosage
Drug Implants chemistry
Fibroblast Growth Factor 2 chemistry
Fracture Healing drug effects
Fracture Healing physiology
Fractures, Malunited diagnosis
Fractures, Malunited physiopathology
Fractures, Malunited therapy
Male
Mice
Mice, Inbred C57BL
Protein Binding
Rats
Treatment Outcome
Bone Transplantation instrumentation
Collagen chemistry
Femoral Fractures physiopathology
Femoral Fractures therapy
Fibroblast Growth Factor 2 administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1748-605X
- Volume :
- 9
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biomedical materials (Bristol, England)
- Publication Type :
- Academic Journal
- Accession number :
- 24844188
- Full Text :
- https://doi.org/10.1088/1748-6041/9/3/035014