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CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria.

Authors :
Lau LS
Fernandez-Ruiz D
Mollard V
Sturm A
Neller MA
Cozijnsen A
Gregory JL
Davey GM
Jones CM
Lin YH
Haque A
Engwerda CR
Nie CQ
Hansen DS
Murphy KM
Papenfuss AT
Miles JJ
Burrows SR
de Koning-Ward T
McFadden GI
Carbone FR
Crabb BS
Heath WR
Source :
PLoS pathogens [PLoS Pathog] 2014 May 22; Vol. 10 (5), pp. e1004135. Date of Electronic Publication: 2014 May 22 (Print Publication: 2014).
Publication Year :
2014

Abstract

To follow the fate of CD8+ T cells responsive to Plasmodium berghei ANKA (PbA) infection, we generated an MHC I-restricted TCR transgenic mouse line against this pathogen. T cells from this line, termed PbT-I T cells, were able to respond to blood-stage infection by PbA and two other rodent malaria species, P. yoelii XNL and P. chabaudi AS. These PbT-I T cells were also able to respond to sporozoites and to protect mice from liver-stage infection. Examination of the requirements for priming after intravenous administration of irradiated sporozoites, an effective vaccination approach, showed that the spleen rather than the liver was the main site of priming and that responses depended on CD8α+ dendritic cells. Importantly, sequential exposure to irradiated sporozoites followed two days later by blood-stage infection led to augmented PbT-I T cell expansion. These findings indicate that PbT-I T cells are a highly versatile tool for studying multiple stages and species of rodent malaria and suggest that cross-stage reactive CD8+ T cells may be utilized in liver-stage vaccine design to enable boosting by blood-stage infections.

Details

Language :
English
ISSN :
1553-7374
Volume :
10
Issue :
5
Database :
MEDLINE
Journal :
PLoS pathogens
Publication Type :
Academic Journal
Accession number :
24854165
Full Text :
https://doi.org/10.1371/journal.ppat.1004135