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Fate mapping reveals origin and dynamics of lymph node follicular dendritic cells.
- Source :
-
The Journal of experimental medicine [J Exp Med] 2014 Jun 02; Vol. 211 (6), pp. 1109-22. Date of Electronic Publication: 2014 May 26. - Publication Year :
- 2014
-
Abstract
- Follicular dendritic cells (FDCs) regulate B cell function and development of high affinity antibody responses but little is known about their biology. FDCs associate in intricate cellular networks within secondary lymphoid organs. In vitro and ex vivo methods, therefore, allow only limited understanding of the genuine immunobiology of FDCs in their native habitat. Herein, we used various multicolor fate mapping systems to investigate the ontogeny and dynamics of lymph node (LN) FDCs in situ. We show that LN FDC networks arise from the clonal expansion and differentiation of marginal reticular cells (MRCs), a population of lymphoid stromal cells lining the LN subcapsular sinus. We further demonstrate that during an immune response, FDCs accumulate in germinal centers and that neither the recruitment of circulating progenitors nor the division of local mature FDCs significantly contributes to this accumulation. Rather, we provide evidence that newly generated FDCs also arise from the proliferation and differentiation of MRCs, thus unraveling a critical function of this poorly defined stromal cell population.<br /> (© 2014 Jarjour et al.)
- Subjects :
- Animals
Cell Differentiation immunology
Cell Proliferation
Dendritic Cells, Follicular metabolism
Germinal Center cytology
Germinal Center metabolism
Luminescent Proteins genetics
Luminescent Proteins metabolism
Lymph Nodes cytology
Lymph Nodes metabolism
Mesoderm cytology
Mesoderm immunology
Mesoderm metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Microscopy, Confocal
RANK Ligand immunology
RANK Ligand metabolism
Receptors, Complement 3b immunology
Receptors, Complement 3b metabolism
Receptors, Complement 3d immunology
Receptors, Complement 3d metabolism
Stem Cells immunology
Stem Cells metabolism
Stromal Cells immunology
Stromal Cells metabolism
Cell Lineage immunology
Dendritic Cells, Follicular immunology
Germinal Center immunology
Lymph Nodes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1540-9538
- Volume :
- 211
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 24863064
- Full Text :
- https://doi.org/10.1084/jem.20132409