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Stem cell treatment after cerebral ischemia regulates the gene expression of apoptotic molecules.
- Source :
-
Neurochemical research [Neurochem Res] 2014 Aug; Vol. 39 (8), pp. 1511-21. Date of Electronic Publication: 2014 May 31. - Publication Year :
- 2014
-
Abstract
- Evidence suggests that apoptosis contributes significantly to cell death after cerebral ischemia. Our recent studies that utilized human umbilical cord blood-derived mesenchymal stem cells (hUCBSCs) demonstrated the potential of hUCBSCs to inhibit neuronal apoptosis in a rat model of CNS injury. Therefore, we hypothesize that intravenous administration of hUCBSCs after focal cerebral ischemia would reduce brain damage by inhibiting apoptosis and downregulating the upregulated apoptotic pathway molecules. Male Sprague-Dawley rats were obtained and randomly assigned to various groups. After the animals reached a desired weight, they were subjected to a 2 h middle cerebral artery occlusion (MCAO) procedure followed by 7 days of reperfusion. The hUCBSCs were obtained, cultured, and intravenously injected (0.25 × 10(6) cells or 1 × 10(6) cells) via the tail vein to separate groups of animals 24 h post-MCAO procedure. We performed various techniques including PCR microarray, hematoxylin and eosin, and TUNEL staining in addition to immunoblot and immunofluorescence analysis in order to investigate the effect of our treatment on regulation of apoptosis after focal cerebral ischemia. Most of the apoptotic pathway molecules which were upregulated after focal cerebral ischemia were downregulated after hUCBSCs treatment. Further, the staining techniques revealed a prominent reduction in brain damage and the extent of apoptosis at even the lowest dose of hUCBSCs tested in the present study. In conclusion, our treatment with hUCBSCs after cerebral ischemia in the rodent reduces brain damage by inhibiting apoptosis and downregulating the apoptotic pathway molecules.
- Subjects :
- Animals
Brain Ischemia pathology
Gene Expression Regulation
Human Umbilical Vein Endothelial Cells transplantation
Humans
Male
Rats
Rats, Sprague-Dawley
Treatment Outcome
Apoptosis physiology
Apoptosis Regulatory Proteins biosynthesis
Brain Ischemia metabolism
Brain Ischemia therapy
Stem Cell Transplantation trends
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6903
- Volume :
- 39
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Neurochemical research
- Publication Type :
- Academic Journal
- Accession number :
- 24879430
- Full Text :
- https://doi.org/10.1007/s11064-014-1341-z