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Enhanced immune responses in pigs by DNA vaccine coexpressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus.
- Source :
-
Journal of virological methods [J Virol Methods] 2014 Sep; Vol. 206, pp. 27-37. Date of Electronic Publication: 2014 May 29. - Publication Year :
- 2014
-
Abstract
- The European (EU) type of porcine reproductive and respiratory syndrome virus (PRRSV) has recently emerged in China. In this study, three recombinant DNA vaccines, pVAX1-EU-ORF3-ORF5 (coexpressing EU type PRRSV GP3 and GP5), pVAX1-EU-ORF3 and pVAX1-EU-ORF5, were constructed and evaluated for their abilities to induce humoral and cellular responses as well as to protect piglets against homologous virus challenge. All piglets were given booster vaccinations at 21 days after the initial inoculation and then challenged 14 days later. Pigs inoculated with pVAX1-EU-ORF3-ORF5 developed significantly higher (P<0.05) PRRSV-specific antibody responses, neutralizing antibodies and levels of IL-4 and IL-10 than those given pVAX1-EU-ORF3, pVAX1-EU-ORF5 or pVAX1. Moreover, pigs immunized with pVAX1-EU-ORF3-ORF5 had markedly increased levels of IFN-γ and IL-2 in serum and T-lymphocytes (CD3(+)CD4(+) and CD3(+)CD8(+) T cells) in peripheral blood. Thus, EU-type PRRSV GP3 and GP5 proteins demonstrated good immunogenicity and reactogenicity and could induce cellular immunity in pigs. Following challenge with the Lelystad virus (LV) strain, piglets inoculated with pVAX1-EU-ORF3-ORF5 showed viremia and virus load distributed in organ tissues that were significantly lower (P<0.05) than those in the pVAX1-EU-ORF3 group and control group, and slightly lower than those in the pVAX1-EU-ORF5 group (P>0.05). As GP3 could enhance humoral- and cell-mediated immune responses to GP5, the results of this study suggested that these two proteins delivered by a vaccine can synergistically induce immunity against PRRSV.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Animal Structures virology
Animals
Antibodies, Neutralizing blood
Antibodies, Viral blood
China
Cytokines blood
Cytokines metabolism
Glycoproteins genetics
Porcine Reproductive and Respiratory Syndrome immunology
Porcine respiratory and reproductive syndrome virus genetics
Swine
T-Lymphocytes immunology
Vaccines, DNA administration & dosage
Vaccines, DNA genetics
Vaccines, Synthetic administration & dosage
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Viral Load
Viral Proteins genetics
Viral Vaccines administration & dosage
Viral Vaccines genetics
Glycoproteins immunology
Porcine Reproductive and Respiratory Syndrome prevention & control
Porcine respiratory and reproductive syndrome virus immunology
Vaccination methods
Vaccines, DNA immunology
Viral Proteins immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0984
- Volume :
- 206
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 24882496
- Full Text :
- https://doi.org/10.1016/j.jviromet.2014.05.021