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PHYTOALEXIN DEFICIENT 4 affects reactive oxygen species metabolism, cell wall and wood properties in hybrid aspen (Populus tremula L. × tremuloides).
- Source :
-
Plant, cell & environment [Plant Cell Environ] 2015 Jul; Vol. 38 (7), pp. 1275-84. Date of Electronic Publication: 2014 Jul 22. - Publication Year :
- 2015
-
Abstract
- The phytoalexin deficient 4 (PAD4) gene in Arabidopsis thaliana (AtPAD4) is involved in the regulation of plant--pathogen interactions. The role of PAD4 in woody plants is not known; therefore, we characterized its function in hybrid aspen and its role in reactive oxygen species (ROS)-dependent signalling and wood development. Three independent transgenic lines with different suppression levels of poplar PAD expression were generated. All these lines displayed deregulated ROS metabolism, which was manifested by an increased H2O2 level in the leaves and shoots, and higher activities of manganese superoxide dismutase (MnSOD) and catalase (CAT) in the leaves in comparison to the wild-type plants. However, no changes in non-photochemical quenching (NPQ) between the transgenic lines and wild type were observed in the leaves. Moreover, changes in the ROS metabolism in the pad4 transgenic lines positively correlated with wood formation. A higher rate of cell division, decreased tracheid average size and numbers, and increased cell wall thickness were observed. The results presented here suggest that the Populus tremula × tremuloides PAD gene might be involved in the regulation of cellular ROS homeostasis and in the cell division--cell death balance that is associated with wood development.<br /> (© 2014 John Wiley & Sons Ltd.)
- Subjects :
- Catalase metabolism
Cell Wall metabolism
Chlorophyll metabolism
Chlorophyll A
Hybridization, Genetic
Lignin analysis
Plant Leaves genetics
Plant Leaves growth & development
Plant Leaves physiology
Plant Proteins genetics
Plant Proteins metabolism
Plant Shoots genetics
Plant Shoots growth & development
Plant Shoots physiology
Plants, Genetically Modified
Populus growth & development
Populus physiology
Reactive Oxygen Species metabolism
Superoxide Dismutase metabolism
Wood genetics
Wood growth & development
Wood physiology
Phytoalexins
Gene Expression Regulation, Plant
Populus genetics
Sesquiterpenes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-3040
- Volume :
- 38
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Plant, cell & environment
- Publication Type :
- Academic Journal
- Accession number :
- 24943986
- Full Text :
- https://doi.org/10.1111/pce.12388