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Tissue-specific expressed antibody variable gene repertoires.
Tissue-specific expressed antibody variable gene repertoires.
- Source :
-
PloS one [PLoS One] 2014 Jun 23; Vol. 9 (6), pp. e100839. Date of Electronic Publication: 2014 Jun 23 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Recent developments in genetic technologies allow deep analysis of the sequence diversity of immune repertoires, but little work has been reported on the architecture of immune repertoires in mucosal tissues. Antibodies are the key to prevention of infections at the mucosal surface, but it is currently unclear whether the B cell repertoire at mucosal surfaces reflects the dominant antibodies found in the systemic compartment or whether mucosal tissues harbor unique repertoires. We examined the expressed antibody variable gene repertoires from 10 different human tissues using RNA samples derived from a large number of individuals. The results revealed that mucosal tissues such as stomach, intestine and lung possess unique antibody gene repertoires that differed substantially from those found in lymphoid tissues or peripheral blood. Mutation frequency analysis of mucosal tissue repertoires revealed that they were highly mutated, with little evidence for the presence of naïve B cells, in contrast to blood. Mucosal tissue repertoires possessed longer heavy chain complementarity determining region 3 loops than lymphoid tissue repertoires. We also noted a large increase in frequency of both insertions and deletions in the small intestine antibody repertoire. These data suggest that mucosal immune repertoires are distinct in many ways from the systemic compartment.
- Subjects :
- Adolescent
Adult
Antibodies blood
Bone Marrow metabolism
Cluster Analysis
Complementarity Determining Regions genetics
DNA metabolism
Demography
Germ Cells metabolism
High-Throughput Nucleotide Sequencing
Humans
Immunoglobulin Variable Region blood
Lymphoid Tissue metabolism
Middle Aged
Mucous Membrane metabolism
Mutation genetics
Mutation Rate
RNA genetics
RNA metabolism
Somatic Hypermutation, Immunoglobulin genetics
V(D)J Recombination genetics
Young Adult
Antibodies genetics
Gene Expression Regulation
Immunoglobulin Variable Region genetics
Organ Specificity genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24956460
- Full Text :
- https://doi.org/10.1371/journal.pone.0100839