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Inhibition of lung tumor growth in nude mice by siRNA CD31 targeting PECAM-1.
- Source :
-
Oncology letters [Oncol Lett] 2014 Jul; Vol. 8 (1), pp. 33-40. Date of Electronic Publication: 2014 Apr 25. - Publication Year :
- 2014
-
Abstract
- Small interfering RNA (siRNA) provides a promising therapeutic approach in the silencing of disease-causing genes. In the present study, the use of 2'-O-methyl-modified siRNA-cluster of differentiation 31 (siRNA <superscript>CD31</superscript> ), with cationic liposome RNA interference (RNAi)-mate as a carrier, effectively silenced the platelet endothelial cell molecule 1 (PECAM-1) gene of murine hemangioendothelioma cells in vitro. In vivo , 2'-O-methyl-modified siRNA <superscript>CD31</superscript> carried by RNAi-mate was successfully delivered, targeting the PECAM-1 gene in the vasculature of nude mouse lung carcinoma xenografts. The growth of the lung carcinoma xenografts was inhibited by the 2'-O-methyl-modified siRNA <superscript>CD31</superscript> and RNAi-mate complexes, and the expression of the PECAM-1 protein was downregulated, with a simultaneous decrease in vascular endothelial growth factor (VEGF) protein in the lung carcinoma xenografts. 2'-O-methyl-modified siRNA <superscript>CD31</superscript> -RNAi-mate complexes may provide a potential therapeutic strategy in lung carcinoma treatment. The effect of PECAM-1 on VEGF expression may possibly be attributed to the function of PECAM-1 signal transduction.
Details
- Language :
- English
- ISSN :
- 1792-1074
- Volume :
- 8
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Oncology letters
- Publication Type :
- Academic Journal
- Accession number :
- 24959215
- Full Text :
- https://doi.org/10.3892/ol.2014.2091