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Virus entry. Lassa virus entry requires a trigger-induced receptor switch.
- Source :
-
Science (New York, N.Y.) [Science] 2014 Jun 27; Vol. 344 (6191), pp. 1506-10. - Publication Year :
- 2014
-
Abstract
- Lassa virus spreads from a rodent to humans and can lead to lethal hemorrhagic fever. Despite its broad tropism, chicken cells were reported 30 years ago to resist infection. We found that Lassa virus readily engaged its cell-surface receptor α-dystroglycan in avian cells, but virus entry in susceptible species involved a pH-dependent switch to an intracellular receptor, the lysosome-resident protein LAMP1. Iterative haploid screens revealed that the sialyltransferase ST3GAL4 was required for the interaction of the virus glycoprotein with LAMP1. A single glycosylated residue in LAMP1, present in susceptible species but absent in birds, was essential for interaction with the Lassa virus envelope protein and subsequent infection. The resistance of Lamp1-deficient mice to Lassa virus highlights the relevance of this receptor switch in vivo.<br /> (Copyright © 2014, American Association for the Advancement of Science.)
- Subjects :
- Amino Acid Sequence
Animals
Cell Line
Cell Membrane metabolism
Cell Membrane virology
Cells, Cultured
Chickens
Dystroglycans genetics
Dystroglycans metabolism
Glycosylation
Humans
Hydrogen-Ion Concentration
Lassa Fever virology
Lysosomal-Associated Membrane Protein 1 chemistry
Lysosomes metabolism
Lysosomes virology
Mice
Mice, Knockout
Molecular Sequence Data
Protein Binding
Sialyltransferases metabolism
beta-Galactoside alpha-2,3-Sialyltransferase
Lassa virus physiology
Lysosomal-Associated Membrane Protein 1 metabolism
Receptors, Virus metabolism
Viral Envelope Proteins metabolism
Virus Internalization
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 344
- Issue :
- 6191
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 24970085
- Full Text :
- https://doi.org/10.1126/science.1252480