Back to Search
Start Over
Cysteine-rich secretory protein 3 inhibits hepatitis C virus at the initial phase of infection.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2014 Jul 25; Vol. 450 (2), pp. 1076-82. Date of Electronic Publication: 2014 Jun 27. - Publication Year :
- 2014
-
Abstract
- Hepatitis C virus (HCV) affects 2-3% of the global population. Approximately one-quarter of acute infections cause chronic hepatitis that leads to liver cirrhosis or hepatocellular carcinoma. The major obstacle of current research is the extremely narrow host tropism of HCV. A single HCV strain can replicate in the Huh7 human hepatoma cell line. Huh7 cells can be adapted under selective pressure in vitro to identify host factors that influence viral replication. Here, we extended this strategy to the in vivo condition and generated a series of cell lines by multiple rounds of adaptation in immunocompromised mice. Adaptation increased the cellular resistance to HCV infection. Microarray analyses revealed that the expression levels of several genes were associated with HCV resistance. Notably, up-regulation of the mRNA encoding cysteine-rich secretory protein 3 (CRISP3), a glycoprotein with unknown function that is secreted from multiple exocrine glands, was correlated with HCV resistance. The presence of CRISP3 in the culture medium limited HCV replication at the early phase of infection.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Carcinoma, Hepatocellular
Cell Line, Tumor cytology
Culture Media
HEK293 Cells
Heterografts
Host-Pathogen Interactions
Humans
Mice, Inbred BALB C
Mice, Nude
Mice, SCID
Neoplasm Transplantation
Recombinant Proteins genetics
Recombinant Proteins metabolism
Salivary Proteins and Peptides genetics
Seminal Plasma Proteins genetics
Virus Replication
Cell Line, Tumor virology
Hepacivirus physiology
Salivary Proteins and Peptides metabolism
Seminal Plasma Proteins metabolism
Virus Internalization
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 450
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 24978310
- Full Text :
- https://doi.org/10.1016/j.bbrc.2014.06.106