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Same-day subtyping of Campylobacter jejuni and C. coli isolates by use of multiplex ligation-dependent probe amplification-binary typing.

Authors :
Cornelius AJ
Vandenberg O
Robson B
Gilpin BJ
Brandt SM
Scholes P
Martiny D
Carter PE
van Vught P
Schouten J
On SL
Source :
Journal of clinical microbiology [J Clin Microbiol] 2014 Sep; Vol. 52 (9), pp. 3345-50. Date of Electronic Publication: 2014 Jul 02.
Publication Year :
2014

Abstract

Campylobacteriosis is the most commonly reported form of human bacterial gastroenteritis in the world. Sound identification of infectious sources requires subtyping, but the most widely used methods have turnaround times measured in days and require specialist equipment and skills. A multiplex ligation-dependent probe amplification-binary typing (MBiT) assay was developed for subtyping Campylobacter jejuni and Campylobacter coli. It was tested on 245 isolates, including recent isolates from Belgium and New Zealand, and compared to multilocus sequence typing (MLST). When used in an outbreak setting, MBiT identified the predominant genotype and possible additional cases days before pulsed-field gel electrophoresis (PFGE) results were available. MBiT was more discriminatory than MLST and, being a single assay with results produced within 6 h, was more rapid and cost-effective than both MLST and PFGE. In addition, MBiT requires only basic molecular biology equipment and skills.<br /> (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)

Details

Language :
English
ISSN :
1098-660X
Volume :
52
Issue :
9
Database :
MEDLINE
Journal :
Journal of clinical microbiology
Publication Type :
Academic Journal
Accession number :
24989612
Full Text :
https://doi.org/10.1128/JCM.00815-14