Back to Search
Start Over
Magnetic single-walled carbon nanotubes-dispersive solid-phase extraction method combined with liquid chromatography-tandem mass spectrometry for the determination of paraquat in urine.
- Source :
-
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2014 Aug 15; Vol. 965, pp. 85-90. Date of Electronic Publication: 2014 Jun 23. - Publication Year :
- 2014
-
Abstract
- In this study, magnetic single-walled carbon nanotubes (MSWCNTs) were prepared by impregnating magnetic Fe3O4 nanoparticles onto the surfaces of carboxylic single-walled carbon nanotubes based on electrostatic interactions. The prepared MSWCNTs were used as the adsorbent for the dispersive solid-phase extraction (DSPE) of paraquat from human urine. After adsorption, the paraquat was quantitatively desorbed with 5%TFA in acetonitrile and determined by HPLC-MS. Extraction parameters such as the type of CNT adsorbent, extraction time, sample volume, wash solvent, and the type and volume of desorption solvent were optimized to obtain high DSPE recoveries and extraction efficiencies. Under the optimized conditions, the calibration curve was linear in the range 3.75-375.0 μg/L with a correlation coefficient of 0.999 45. The LOD (S/N=3) and LOQ (S/N=10) were 0.94 and 2.82 μg/L, respectively. The recoveries ranged from 92.89 to 108.9% for spiked real urine samples with RSDs below 3.21%. Finally, the new method was successfully used to determine paraquat in urine samples of suspected paraquat poisoning patients. The MSWCNTs exhibited suitable properties and a high adsorption capacity for the extraction of paraquat.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Humans
Limit of Detection
Linear Models
Paraquat chemistry
Paraquat isolation & purification
Paraquat poisoning
Reproducibility of Results
Tandem Mass Spectrometry methods
Chromatography, Liquid methods
Magnetite Nanoparticles chemistry
Nanotubes, Carbon chemistry
Paraquat urine
Solid Phase Extraction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-376X
- Volume :
- 965
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 24999616
- Full Text :
- https://doi.org/10.1016/j.jchromb.2014.06.016