Back to Search
Start Over
Enzyme inhibition, antioxidant and immunomodulatory activities, and brine shrimp toxicity of extracts from the root bark, stem bark and leaves of Terminalia macroptera.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2014 Sep 11; Vol. 155 (2), pp. 1219-26. Date of Electronic Publication: 2014 Jul 10. - Publication Year :
- 2014
-
Abstract
- Ethnopharmacological Relevance: The root bark, stem bark and leaves of Terminalia macroptera have been traditionally used against a variety of ailments such as wounds, hepatitis, malaria, fever, cough, and diarrhea as well as tuberculosis and skin diseases in African folk medicine. Boiling water extracts of Terminalia macroptera, administered orally, are the most common preparations of this plant used by the traditional healers in Mali. This study aimed to investigate the inhibition of the activities of α-glucosidase, 15-lipoxygenase and xanthine oxidase, DPPH scavenging activity, complement fixation activity and brine shrimp toxicity of different extracts obtained by boiling water extraction (BWE) and by ASE (accelerated solvent extraction) with ethanol, ethanol-water and water as extractants from different plant parts of Terminalia macroptera.<br />Materials and Methods: 27 different crude extracts were obtained by BWE and ASE from root bark, stem bark and leaves of Terminalia macroptera. The total phenolic and carbohydrate contents, enzyme inhibition activities (α-glucosidase, 15-lipoxygenase and xanthine oxidase), DPPH scavenging activity, complement fixation activity and brine shrimp toxicity of these extracts were evaluated. Principal component analysis (PCA) was applied for total biological activities evaluation.<br />Results: Several of the extracts from root bark, stem bark and leaves of Terminalia macroptera obtained by BWE and ASE showed potent enzyme inhibition activities, radical-scavenging properties and complement fixation activities. None of the extracts are toxic against brine shrimp larvae in the test concentration. Based on the results from PCA, the ASE ethanol extracts of root bark and stem bark and the low molecular weight fraction of the 50% ethanol-water extract of leaves showed the highest total biological activities. The boiling water extracts were less active, but the bark extracts showed activity as α-glucosidase inhibitors and radical scavengers, the leaf extract being less active.<br />Conclusion: The observed enzyme inhibition activities, radical scavenging properties and complement fixation activities may explain some of the traditional uses of this medicinal tree, such as in wound healing and against diabetes.<br /> (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Antioxidants chemistry
Antioxidants isolation & purification
Antioxidants toxicity
Biphenyl Compounds chemistry
Complement Activation drug effects
Complement Fixation Tests
Enzyme Inhibitors chemistry
Enzyme Inhibitors isolation & purification
Enzyme Inhibitors toxicity
Glycoside Hydrolase Inhibitors pharmacology
Immunologic Factors chemistry
Immunologic Factors isolation & purification
Immunologic Factors toxicity
Lethal Dose 50
Lipoxygenase Inhibitors pharmacology
Phytotherapy
Picrates chemistry
Plant Bark
Plant Extracts chemistry
Plant Extracts isolation & purification
Plant Extracts toxicity
Plant Leaves
Plant Roots
Plant Stems
Plants, Medicinal
Principal Component Analysis
Sheep
Xanthine Oxidase antagonists & inhibitors
Xanthine Oxidase metabolism
Antioxidants pharmacology
Artemia drug effects
Enzyme Inhibitors pharmacology
Immunologic Factors pharmacology
Plant Extracts pharmacology
Terminalia chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 155
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 25017373
- Full Text :
- https://doi.org/10.1016/j.jep.2014.07.004