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Effects of laver extracts on adhesion, invasion, and migration in SK-Hep1 human hepatoma cancer cells.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2014; Vol. 78 (6), pp. 1044-51. Date of Electronic Publication: 2014 May 28. - Publication Year :
- 2014
-
Abstract
- The laver (Porphyra tenera), red seaweed, has been reported to have anticancer activity, but little is known about its molecular mechanisms of action. The objective of this study was to determine the effects of laver extract on cancer cell proliferation, invasion, and metastasis in SK-Hep1 cells using migration and invasion assays. We also investigated the relationship of MMP-2/-9 and TIMP-1/-2 expression at both the protein and gene level in SK-Hep1 human hepatoma carcinoma cells after laver extract treatment. Laver extract inhibited cancer cell growth in a dose-dependent manner. In an invasion assay conducted in Transwell chambers, laver extract showed 19.6 and 27.2% inhibition of cancer cell at 200 and 400 μg/mL, respectively, compared to the control. The mRNA levels of both MMP-2 and MMP-9 were down-regulated by laver extract treatment in a dose-dependent manner. Laver extract, at 400 μg/mL, was inhibited by MMP-2 and MMP-9 expressions by 70.1 and 77.0%, respectively. An inverse relationship in the mRNA contents of MMP-2/-9 and TIMP-1/-2 expressions in SK-Hep1 cells was found by laver extract treatment. Our results demonstrate antimetastatic properties of laver extract in inhibiting the adhesion, invasion, and migration of SK-Hep1 human hepatoma cancer cells.
- Subjects :
- Antineoplastic Agents chemistry
Cell Adhesion drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Chlorophyll analysis
Flavonoids analysis
Gene Expression Regulation, Neoplastic drug effects
Humans
Matrix Metalloproteinase 2 genetics
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 genetics
Matrix Metalloproteinase 9 metabolism
Neoplasm Invasiveness
Phenol analysis
Plant Extracts chemistry
Tissue Inhibitor of Metalloproteinase-1 genetics
Tissue Inhibitor of Metalloproteinase-1 metabolism
Tissue Inhibitor of Metalloproteinase-2 genetics
Tissue Inhibitor of Metalloproteinase-2 metabolism
beta Carotene analysis
Antineoplastic Agents pharmacology
Carcinoma, Hepatocellular pathology
Cell Movement drug effects
Liver Neoplasms pathology
Plant Extracts pharmacology
Porphyra chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 78
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25036133
- Full Text :
- https://doi.org/10.1080/09168451.2014.912116