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Quantitative analysis of CD3ε in a cloned canine lymphoma cell line by selected reaction monitoring assay.

Authors :
Kamiie J
Shimoyama N
Aihara N
Hisasue M
Naya Y
Ogihara K
Shirota K
Source :
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2014; Vol. 78 (2), pp. 271-5. Date of Electronic Publication: 2014 Apr 16.
Publication Year :
2014

Abstract

We established a mass spectrometry-based quantitative method of assaying CD3ε, a component of the T-cell receptor complex. It revealed a CD3ε level of 1 mol per cell in a newly derived canine T-cell lymphoma cell line. Our results suggest that this method has sufficient sensitivity to quantify CD3ε levels in canine lymphoma cells reliably.

Details

Language :
English
ISSN :
1347-6947
Volume :
78
Issue :
2
Database :
MEDLINE
Journal :
Bioscience, biotechnology, and biochemistry
Publication Type :
Academic Journal
Accession number :
25036680
Full Text :
https://doi.org/10.1080/09168451.2014.878216