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Quantitative analysis of CD3ε in a cloned canine lymphoma cell line by selected reaction monitoring assay.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2014; Vol. 78 (2), pp. 271-5. Date of Electronic Publication: 2014 Apr 16. - Publication Year :
- 2014
-
Abstract
- We established a mass spectrometry-based quantitative method of assaying CD3ε, a component of the T-cell receptor complex. It revealed a CD3ε level of 1 mol per cell in a newly derived canine T-cell lymphoma cell line. Our results suggest that this method has sufficient sensitivity to quantify CD3ε levels in canine lymphoma cells reliably.
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 78
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25036680
- Full Text :
- https://doi.org/10.1080/09168451.2014.878216