Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase.

Authors :: Mangan D
McCleary BV
Liadova A
Ivory R
McCormack N
Source :: Carbohydrate research [Carbohydr Res] 2014 Aug 18; Vol. 395, pp. 47-51. Date of Electronic Publication: 2014 May 15.
Publication Year :: 2014
Abstract: There is a growing demand for research tools to aid the scientific community in the search for improved cellulase enzymes for the biofuel industry. In this work, we describe a novel fluorometric assay for cellulase (endo-1,4-β-glucanase) which is based on the use of 4,6-O-benzylidene-4-methylumbelliferyl-β-cellotrioside (BzMUG3) in the presence of an ancillary β-glucosidase. This assay can be used quantitatively over a reasonable linear range, or qualitatively as a solution screening tool which may find extensive use in the area of metagenomics.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)