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Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species.
- Source :
-
Biotechnology letters [Biotechnol Lett] 2014 Nov; Vol. 36 (11), pp. 2311-7. Date of Electronic Publication: 2014 Jul 22. - Publication Year :
- 2014
-
Abstract
- The end products from starch hydrolysis by amylases have important applications in various industries. Here, two amylases derived from two Streptomyces species that hydrolyze soluble starch from potato produced maltotriose as the primary maltooligosaccharide product. The genes, annotated as putative glycoside hydrolases, were cloned and expressed in Streptomyces lividans. These amylases displayed hydrolysis activity from pH 3 to 9.5 and were not affected by Ca(2+.) Optimal production of maltotriose was between 20 and 30 °C at pH 6.5. At the optimal temperature, both amylases produced maltotriose-rich end products rather than either maltose or maltotetraose.
- Subjects :
- Amylases chemistry
Amylases genetics
Chromatography, High Pressure Liquid
Cloning, Molecular
Hydrogen-Ion Concentration
Hydrolysis
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Streptomyces genetics
Temperature
Amylases metabolism
Starch metabolism
Streptomyces enzymology
Trisaccharides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6776
- Volume :
- 36
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Biotechnology letters
- Publication Type :
- Academic Journal
- Accession number :
- 25048235
- Full Text :
- https://doi.org/10.1007/s10529-014-1611-5