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Sequential expression of long noncoding RNA as mRNA gene expression in specific stages of mouse spermatogenesis.
- Source :
-
Scientific reports [Sci Rep] 2014 Aug 06; Vol. 4, pp. 5966. Date of Electronic Publication: 2014 Aug 06. - Publication Year :
- 2014
-
Abstract
- Many long noncoding RNA (lncRNA) species have been identified in gametes. However, the biogenesis and function of other categories of lncRNAs in gametes is poorly understood. Here, we profiled the expression of lncRNAs and mRNAs in spermatogonial stem cells (SSC), type A spermatogonia (A), pachytene spermatocytes (PS) and round spermatids (RS) by microarray analysis. We analyze the total expression of lncRNA/mRNA in these four germ cells and found that the maximum number of lncRNAs expression is in A (22127), and the minimum is in PS (14456). Also, the maximum number of mRNAs is in A (19923), and the minimum is in PS (13941). Furthermore, the trend in the number of specific lncRNAs was similar to the number of specific mRNAs in each type of germ cells (e.g., maximum in A and minimum in PS). The trend in the number of lncRNAs was similar to the number of mRNAs in two continued types of germ cells (e.g., maximum in SSC to A and minimum in PS to RS). The correlation analysis showed a high correlation coefficient of lncRNAs/mRNAs expression (R = 0.992). The results suggested that the sequential expression of long noncoding RNA as mRNA gene expression exhibits coordinated changes in male spermatogenesis.
- Subjects :
- Adult Stem Cells cytology
Adult Stem Cells metabolism
Animals
Gene Expression Profiling
Genetic Markers
Male
Mice
Mice, Inbred C57BL
Microarray Analysis
RNA, Long Noncoding metabolism
RNA, Messenger metabolism
Spermatids cytology
Spermatids growth & development
Spermatids metabolism
Spermatocytes cytology
Spermatocytes growth & development
Spermatocytes metabolism
Spermatogonia cytology
Spermatogonia growth & development
Spermatogonia metabolism
Gene Expression Regulation, Developmental
RNA, Long Noncoding genetics
RNA, Messenger genetics
Spermatogenesis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 4
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 25097017
- Full Text :
- https://doi.org/10.1038/srep05966