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The involvement of cysteine-rich intestinal protein in early development and innate immunity of Asiatic hard clam, Meretrix meretrix.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2014 Oct; Vol. 40 (2), pp. 435-40. Date of Electronic Publication: 2014 Aug 06. - Publication Year :
- 2014
-
Abstract
- Cysteine-rich intestinal protein (CRIP), a Zn(2+)-binding protein, contains a single copy of the highly conserved double-zinc-finger structure known as the LIM (lin-11-isl-1-mec-3) motif. In this paper, a cDNA encoding MmCRIP was isolated from the Asiatic hard clam Meretrix meretrix. The full-length cDNA of MmCRIP consists of a 237-bp open reading frame that encodes a polypeptide of 78 amino acids with a predicted molecular weight (MW) of 8635.8 Da and theoretical isoelectric point (pI) of 9.01. Bioinformatics analysis showed that it belonged to a new member of the CRIP subfamily. Relationship analysis revealed that MmCRIP has high-levels of sequence similarity to many CRIPs reported in other animals, particularly in invertebrates. Real-time PCR analysis showed that the highest level of MmCRIP expression was in hemocyte tissue and at pediveligers stage. To investigate immune function, mature clams were challenged with Aeromonas hydrophila. During A. hydrophila infection, up-regulation of MmCRIP transcript in clam's hemocyte, gill and hepatopancreas was detected. DsRNAi (double-strand RNA interference) approach was employed to study the function of MmCRIP and the data showed that inactivation of the MmCRIP gene blocked larvae development and caused mass mortalities. The probable roles of MmCRIP in clam early development and innate immunity are presented for the first time.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Aeromonas hydrophila physiology
Amino Acid Sequence
Animals
Base Sequence
Bivalvia growth & development
Bivalvia immunology
Bivalvia microbiology
DNA, Complementary genetics
DNA, Complementary metabolism
Immunity, Innate
LIM Domain Proteins chemistry
LIM Domain Proteins metabolism
Larva genetics
Larva growth & development
Larva immunology
Larva microbiology
Molecular Sequence Data
Phylogeny
RNA Interference
Real-Time Polymerase Chain Reaction
Sequence Alignment
Bivalvia genetics
LIM Domain Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 40
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25108085
- Full Text :
- https://doi.org/10.1016/j.fsi.2014.07.027