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The application of metabolite profiling to Mycobacterium spp.: determination of metabolite changes associated with growth.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2014 Nov; Vol. 106, pp. 23-32. Date of Electronic Publication: 2014 Aug 07. - Publication Year :
- 2014
-
Abstract
- In order to decipher the complex biological networks underlying biochemical and physiological processes, cellular regulation at all levels must be studied. The metabolites determined by metabolomics represent the end-point of cellular regulation and thus vital components of any integrative network. In the case of pathogenic agents such as Mycobacterium tuberculosis metabolomics offers an ideal opportunity to gain a better understanding of how this species adapts to environmental conditions and antimicrobial treatments. In the present study a metabolite profiling protocol for Mycobacterium including optimised quenching, extraction and analysis has been devised. These methods have been applied to three different Mycobacterium spp. demonstrating potential translation across the genus. Steady-state levels of metabolites during growth have been determined for Mycobacterium smegmatis, Mycobacterium phlei and Mycobacterium bovis BCG (Bacillus Calmette-Guérin). The changes of designated biomarkers emphasised phenotypical differences (e.g. nitrogen metabolism) and similarities (e.g. cysteine biosynthesis) between the bacteria. Each time point showed distinguishable metabolic characteristics from early lag to late stationary phase/beginning of non-replicating phase. The combination of the metabolic results with published "omics" data indicated that transcription appeared to be the most predominant mode of cellular regulation utilised by these bacteria studied.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Metabolomics methods
Mycobacterium bovis chemistry
Mycobacterium phlei chemistry
Mycobacterium smegmatis chemistry
Time Factors
Metabolome
Mycobacterium bovis growth & development
Mycobacterium bovis metabolism
Mycobacterium phlei growth & development
Mycobacterium phlei metabolism
Mycobacterium smegmatis growth & development
Mycobacterium smegmatis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8359
- Volume :
- 106
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 25108291
- Full Text :
- https://doi.org/10.1016/j.mimet.2014.07.037